Arsenal midfielder Aaron Ramsey is out of Wales' squad for October's World Cup qualifiers against Austria and Georgia because of a hamstring injury.
Ramsey, 25, has not played since suffering the injury on the opening weekend of the Premier League season.
Midfielder Jonny Williams has returned to light training with loan club Ipswich but is lacking match fitness and is also not included.
Shaun MacDonald has been called up, but George Williams is left out.
The Euro 2016 semi-finalists started their World Cup qualifying campaign with a 4-0 win over Moldova in September and play Austria on 6 October followed by Georgia in Cardiff three days later.
Ramsey was Wales' outstanding player at Euro 2016, but was suspended for the 2-0 semi-final defeat by Portugal.